- PRODUCT DESCRIPTION
+ Inherited gene defects related to the coagulation system have been reported as risk factors for stroke.
+ Recently, a genetic component in the factor V (FV) gene that contributes to activated protein C resistance both in the presence and absence of FV 1691 GA was reported.
+ This highly conserved FV gene haplotype was marked as R2 polymorphism, an A to G alteration at position 4070 in exon 13 that predicts the His 1299 Arg substitutions.
- PRINCIPLE OF THE SYSTEM
+ During the PCR reaction, the DNA polymerase cleaves the probe at the 5’ end
+ Separates the reporter dye from the quencer dye only when the probe hybridizes perfectly to the target DNA.
+ This cleavage results in the fluorescent signal which is monitored by Real-Time PCR detection system.
+ An increase in the fluorescent signal (CT) is proportional to the amount of the specific PCR product.
